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05/12/2016 Metabolic brainstorming meeting (Edda, Judith, Laura, Timo, Roman, Katja)
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AIM: Plan for integration of the new metabolic dataset
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### Dataset
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- ~15 timepoints during one cell cycle, elutriated cells
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- [Metabolomics](met-data) of the central metabolism, amino acids, nucleotides; not compartment resolved
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- [Proteomics](prot-data), proteome wide
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- [Concentrations of metabolites](met-data) in the external medium
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- [Cell volume](vol-data) over time
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- [Literature values](literature-values)
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### Plan for Integration
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1. Map measured metabolites to Y7, generate reaction network
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2. Calculate fluxes between the mapped metabolites with FBA (pFBA) and external uptake rates
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3. For each reaction, fit kinetic parameters for all 15 steady states with given flux, enzyme and metabolite concentrations
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### What can happen?
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1. Flux changes **can** be explained by changes in enzyme and metabolite concentrations with same parameters -> no regulation
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2. Flux changes **cannot** be explained by changes in enzyme and metabolite concentrations with same parameters -> regulation required |
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